Human NKIRAS2(NF-kappa-B inhibitor-interacting Ras-like protein 2) ELISA Kit
Alternative Names
NKIRAS2 ELISA Kit, NF-kappa-B inhibitor-interacting Ras-like protein 2 ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
0.313-20ng/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of NKIRAS2 and the recovery rates were calculated by comparing the measured value to the expected amount of NKIRAS2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-100
96
EDTA plasma(n=5)
90-105
98
heparin plasma(n=5)
95-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKIRAS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-104%
86-105%
89-105%
EDTA plasma(n=5)
90-97%
83-99%
84-99%
heparin plasma(n=5)
90-100%
82-100%
85-97%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
NKIRAS2 ELISA Kit allows for the in vitro quantitative determination of NKIRAS2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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