Human Nitric Oxide Synthase-interacting Protein

Mfr.No.

E6495Hu

Sensitivity

0.029ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CGI-25;E3 ubiquitin-protein ligase NOSIP;eNOS-interacting protein;Nitric oxide Synthase-interacting protein;NOSIP;RING-type E3 ubiquitin transferase NOSIP

Molecular Weight

33172

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6495Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.4	0.34	4.01
Intra-Assay	2	18	0.86	0.06	6.63
Intra-Assay	3	18	2.51	0.15	6.13

Background

E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).

Protocol

These standard curves of E6495Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.109	2.177	2.117
	2.246
8ng/ml	1.626	1.683	1.623
	1.739
4ng/ml	1.054	1.068	1.008
	1.082
2ng/ml	0.784	0.775	0.715
	0.766
1ng/ml	0.404	0.416	0.356
	0.427
0ng/ml	0.065	0.06	0
	0.055

Gene ID	51070
UniProt ID	Q9Y314

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115395	E6495Hu	48T	$320		Add to Cart
BHE12115395	E6495Hu	96T	$458		Add to Cart

Human Nitric Oxide Synthase-interacting Protein - NOSIP ELISA Kit

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