Matrices listed below were spiked with certain level of NIS and the recovery rates were calculated by comparing the measured value to the expected amount of NIS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
93
EDTA plasma(n=5)
88-104
98
heparin plasma(n=5)
87-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NIS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
94-102%
92-104%
EDTA plasma(n=5)
86-100%
86-95%
85-97%
heparin plasma(n=5)
86-99%
87-98%
90-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q92911
Applications
ELISA
Applications Notes
NIS ELISA Kit allows for the in vitro quantitative determination of NIS concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human NIS(Sodium/iodide cotransporter) ELISA Kit