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Home / ELISA Kit

Human Ninein (NIN) ELISA Kit

SKU : BHE13703551

NIN encodes one of the proteins important for centrosomal function. Ninein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

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Mfr.No.	KTE61335
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Ninein (NIN) ELISA Kit employs a two-site sandwich ELISA to quantitate NIN.
Alternative Names	NIN; KIAA1565; GSK3B-interacting protein; glycogen synthase kinase 3 beta-interacting protein; ninein; ninein centrosomal protein
Detection Method	Colorimetric
Kit Components	Human Ninein microplate Human Ninein standard Human Ninein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Ninein (NIN) ELISA Kit has high sensitivity and excellent specificity for detection of Human NIN. No significant cross-reactivity or interference between Human NIN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	NIN encodes one of the proteins important for centrosomal function. Ninein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Notes	This Human Ninein (NIN) ELISA Kit employs a two-site sandwich ELISA to quantitate NIN in samples. An antibody specific for NIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NIN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	51199

Applications	ELISA
Applications Notes	This Human Ninein (NIN) ELISA Kit employs a two-site sandwich ELISA to quantitate NIN in samples. An antibody specific for NIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NIN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703551	KTE61335	48 T	$339		Add to Cart
BHE13703551	KTE61335	96 T	$589		Add to Cart
BHE13703551	KTE61335	96 Tx5	$2629		Add to Cart
BHE13703551	KTE61335	96 Tx50	$24569		Add to Cart

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