Matrices listed below were spiked with certain level of NGB and the recovery rates were calculated by comparing the measured value to the expected amount of NGB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
99
EDTA plasma(n=5)
85-105
97
heparin plasma(n=5)
91-99
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NGB ELISA Kit allows for the in vitro quantitative determination of NGB concentrations in serum, plasma, tissue homogenates and other biological fluids.