Human NGAL/MMP-9 complex ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ01026975HU

Sensitivity

0.029 ng/mL

Detection Range

0.313 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

NGAL/MMP-9 complex

Recovery

The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	91	84-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-95
1:2	Average %	97
	Range %	91-105
1:4	Average %	96
	Range %	92-100
1:8	Average %	93
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	NGAL, MMP-9 complex

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Biomarkers upon discontinuation of renal replacement therapy predict 60╀ay survival and renal recovery in critically ill patients with acute kidney injury Tingting Yang.et al,Hemodialysis International,Jan 11

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