Human Nfkb Repressing Factor

Mfr.No.

E3506Hu

Description

This sandwich kit is for the accurate quantitative detection of human NFKB Repressing Factor (also known as NKRF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NF-kappa-B-repressing factor;NFkB-repressing factor;NKRF;Protein ITBA4;Transcription factor NRF

Reactivity

Human

Detection Range

3-900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

77673

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.47ng/L

Background

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.

Gene ID	55922
UniProt ID	O15226
Antigen	NKRF

Research Area

Others

Protocol

These standard curves of E3506Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.363	2.372	2.325
	2.382
240ng/L	1.525	1.497	1.45
	1.469
120ng/L	0.759	0.796	0.749
	0.834
60ng/L	0.509	0.517	0.47
	0.525
30ng/L	0.245	0.239	0.192
	0.232
0ng/L	0.03	0.047	0
	0.065

Precision

We measured random samples of E3506Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	324	11.46	3.5
Intra-Assay	2	18	91.1	5.57	6.1
Intra-Assay	3	18	130	4.81	3.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105009	E3506Hu	48T	$320		Add to Cart
BHE12105009	E3506Hu	96T	$458		Add to Cart

Human Nfkb Repressing Factor - NKRF ELISA Kit

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