Matrices listed below were spiked with certain level of NFE2L3 and the recovery rates were calculated by comparing the measured value to the expected amount of NFE2L3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-103
98
EDTA plasma(n=5)
94-103
98
heparin plasma(n=5)
89-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFE2L3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-99%
93-105%
85-105%
EDTA plasma(n=5)
86-100%
90-99%
87-101%
heparin plasma(n=5)
83-99%
81-99%
80-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9Y4A8
Applications
ELISA
Applications Notes
NFE2L3 ELISA Kit allows for the in vitro quantitative determination of NFE2L3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human NFE2L3 (Nuclear factor erythroid 2-related factor 3) ELISA Kit