Human Nf-Kappa-B-repressing Factor

Mfr.No.

E5773Hu

Sensitivity

4.25ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

NF-kappa-B-repressing factor;NFkB-repressing factor;NKRF;Protein ITBA4;Transcription factor NRF

Molecular Weight

77673

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5773Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	434.2	17.32	4
Intra-Assay	2	18	677.1	27.22	4
Intra-Assay	3	18	1055.4	27.56	2.6

Background

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.

Protocol

These standard curves of E5773Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.142	2.099	2.038
	2.055
600ng/L	0.957	1.03	0.969
	1.103
300ng/L	0.498	0.537	0.477
	0.576
150ng/L	0.176	0.171	0.111
	0.166
75ng/L	0.124	0.136	0.075
	0.147
0ng/L	0.061	0.06	0
	0.06

Gene ID	55922
UniProt ID	O15226

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107135	E5773Hu	48T	$320		Add to Cart
BHE12107135	E5773Hu	96T	$458		Add to Cart

Human Nf-Kappa-B-repressing Factor - NKRF ELISA Kit

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