Human Nf-Kappa-B Inhibitor Alpha

Mfr.No.

E5772Hu

Sensitivity

14.01ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IkappaBalpha;I-kappa-B-alpha;IkB-alpha;Major histocompatibility complex enhancer-binding protein MAD3;NF-kappa-B inhibitor alpha;NFKBIA

Molecular Weight

35609

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5772Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1628	99.56	6.1
Intra-Assay	2	18	3402.8	120.73	3.5
Intra-Assay	3	18	1017.7	67.02	6.6

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Protocol

These standard curves of E5772Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.255	2.198	2.148
	2.141
2400ng/L	1.384	1.402	1.352
	1.42
1200ng/L	0.751	0.829	0.779
	0.907
600ng/L	0.406	0.467	0.417
	0.529
300ng/L	0.305	0.28	0.23
	0.255
0ng/L	0.05	0.05	0
	0.05

Gene ID	4792
UniProt ID	P25963

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107134	E5772Hu	48T	$320		Add to Cart
BHE12107134	E5772Hu	96T	$458		Add to Cart

Human Nf-Kappa-B Inhibitor Alpha - NFKBIA ELISA Kit

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