Human Neutrophil defensin 3(DEFA3) ELISA kit

Human Neutrophil defensin 3(DEFA3) ELISA kit

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Mfr.No.

CSB-EL006655HU

Sensitivity

0.47 ng/mL

Detection Range

1.87 ng/mL-120 ng/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

defensin, alpha 3, neutrophil-specific, DEFA3 ELISA Kit; DEF3Neutrophil defensin 3 ELISA Kit; Defensin ELISA Kit; alpha 3 ELISA Kit; HNP-3 ELISA Kit; HP-3 ELISA Kit; HP3) (Cleaved into: HP 3-56; Neutrophil defensin 2 ELISA Kit; HNP-2 ELISA Kit; HP-2 ELISA Kit; HP2)) ELISA Kit

Recovery

The recovery of human NR4A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-96
EDTA plasma (n=4)	93	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NR4A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-101
1:2	Average %	95
	Range %	86-106
1:4	Average %	96
	Range %	90-103
1:8	Average %	95
	Range %	82-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.084	2.098	2.091	1.988
1000	1.767	1.786	1.777	1.674
500	1.359	1.322	1.341	1.238
250	0.817	0.828	0.823	0.720
125	0.521	0.534	0.528	0.425
62.5	0.340	0.332	0.336	0.233
31.25	0.285	0.274	0.280	0.177
0	0.104	0.101	0.103

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P59666

Applications	ELISA
Research Area	DEFA3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503564	CSB-EL006655HU	96T	$695	Add to Cart
BHE10503564	CSB-EL006655HU	5x96T	$3336	Add to Cart
BHE10503564	CSB-EL006655HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.