Human Neutrophil Cytosolic Factor 2

Mfr.No.

E3916Hu

Description

This sandwich kit is for the accurate quantitative detection of human Neutrophil Cytosolic Factor 2 (also known as NCF2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

67 kDa neutrophil oxidase factor;NADPH oxidase activator 2;NCF 2;NCF2;NCF-2;Neutrophil cytosol factor 2;Neutrophil NADPH oxidase factor 2;p67-phox

Reactivity

Human

Detection Range

5-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

59762

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.59ng/L

Background

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Gene ID	4688
UniProt ID	P19878
Antigen	NCF2

Research Area

Others

Protocol

These standard curves of E3916Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.07	2.11	2.055
	2.15
400ng/L	1.716	1.729	1.674
	1.743
200ng/L	1.273	1.223	1.168
	1.173
100ng/L	0.656	0.636	0.581
	0.616
50ng/L	0.429	0.409	0.354
	0.389
0ng/L	0.049	0.055	0
	0.062

Precision

We measured random samples of E3916Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	98.5	5.9	6
Intra-Assay	2	18	288	14.56	5.1
Intra-Assay	3	18	160.4	7.74	4.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105354	E3916Hu	48T	$320		Add to Cart
BHE12105354	E3916Hu	96T	$458		Add to Cart

Human Neutrophil Cytosolic Factor 2 - NCF2 ELISA Kit

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