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Home / ELISA Kit

Human Neuron navigator 3 (NAV3) ELISA Kit

SKU : BHE13703573

NAV3 belongs to the neuron navigator family and is expressed predominantly in the nervous system. Neuron navigator 3 contains coiled-coil domains and a conserved AAA domain characteristic for ATPases associated with a variety of cellular activities. NAV3 is similar to unc-53, a Caenorhabditis elegans gene involved in axon guidance. Multiple alternatively spliced transcript variants for NAV3 have been described but only one has had its full-length nature determined.

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Mfr.No.	KTE61358
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Neuron navigator 3 (NAV3) ELISA Kit employs a two-site sandwich ELISA to quantitate NAV3.
Alternative Names	NAV3; KIAA0938; POMFIL1; STEERIN3; unc53H3; pore membrane and/or filament interacting like protein 1; steerin 3
Detection Method	Colorimetric
Kit Components	Human Neuron navigator 3 microplate Human Neuron navigator 3 standard Human Neuron navigator 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Neuron navigator 3 (NAV3) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAV3. No significant cross-reactivity or interference between Human NAV3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	NAV3 belongs to the neuron navigator family and is expressed predominantly in the nervous system. Neuron navigator 3 contains coiled-coil domains and a conserved AAA domain characteristic for ATPases associated with a variety of cellular activities. NAV3 is similar to unc-53, a Caenorhabditis elegans gene involved in axon guidance. Multiple alternatively spliced transcript variants for NAV3 have been described but only one has had its full-length nature determined.
Notes	This Human Neuron navigator 3 (NAV3) ELISA Kit employs a two-site sandwich ELISA to quantitate NAV3 in samples. An antibody specific for NAV3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAV3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAV3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAV3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	89795

Applications	ELISA
Applications Notes	This Human Neuron navigator 3 (NAV3) ELISA Kit employs a two-site sandwich ELISA to quantitate NAV3 in samples. An antibody specific for NAV3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAV3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAV3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAV3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703573	KTE61358	48 T	$339		Add to Cart
BHE13703573	KTE61358	96 T	$589		Add to Cart
BHE13703573	KTE61358	96 Tx5	$2629		Add to Cart
BHE13703573	KTE61358	96 Tx50	$24569		Add to Cart

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