Human Neurogranin - NRGN ELISA Kit

Mfr.No.

E3883Hu

Sensitivity

2.42ng/ml

Detection Range

5-1500ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Neurogranin (also known as NRGN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NEUG(55-78;Neurogranin;Ng;NRGN;RC3

Molecular Weight

7618

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3883Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	500.6	21.93	4.4
Intra-Assay	2	18	162.4	5.87	3.6
Intra-Assay	3	18	332.1	18.02	5.4

Background

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).

Protocol

These standard curves of E3883Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/ml	2.187	2.117	2.072
	2.047
400ng/ml	1.364	1.47	1.425
	1.576
200ng/ml	0.629	0.61	0.565
	0.591
100ng/ml	0.363	0.387	0.342
	0.41
50ng/ml	0.128	0.157	0.112
	0.186
0ng/ml	0.048	0.045	0
	0.041

Gene ID	4900
UniProt ID	Q92686

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105323	E3883Hu	48T	$320		Add to Cart
BHE12105323	E3883Hu	96T	$458		Add to Cart

Human Neurogranin - NRGN ELISA Kit

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