Human Neurogenic Locus Notch Homolog Protein 3

Mfr.No.

E3263Hu

Description

This sandwich kit is for the accurate quantitative detection of human Neurogenic Locus Notch Homolog Protein 3 (also known as NOTCH3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CADASIL;CADASIL1;CASIL;IMF2;LMNS;Neurogenic locus notch homolog protein 3;Notch 3;Notch 3 extracellular truncation;Notch 3 intracellular domain);NOTCH3

Reactivity

Human

Detection Range

70-15000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

243631

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

36.17ng/L

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination (PubMed:15350543). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).

Gene ID	4854
UniProt ID	Q9UM47
Antigen	NOTCH3

Research Area

Neuroscience

Protocol

These standard curves of E3263Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8000ng/L	2.106	2.194	2.151
	2.281
4000ng/L	1.537	1.647	1.604
	1.756
2000ng/L	0.974	1.04	0.998
	1.107
1000ng/L	0.701	0.697	0.655
	0.694
500ng/L	0.428	0.449	0.406
	0.47
0ng/L	0.04	0.043	0
	0.045

Precision

We measured random samples of E3263Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2001.48	75.45	3.77
Intra-Assay	2	18	2810.11	106.45	3.79
Intra-Assay	3	18	455.16	29.79	6.54

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104792	E3263Hu	48T	$320		Add to Cart
BHE12104792	E3263Hu	96T	$458		Add to Cart

Human Neurogenic Locus Notch Homolog Protein 3 - NOTCH3 ELISA Kit

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