Human Neuroblastoma suppressor of tumorigenicity 1(NBL1) ELISA kit

Mfr.No.

CSB-EL015485HU

Alternative Names

neuroblastoma, suppression of tumorigenicity 1, RP5-1056L3.4, D1S1733E, DAN, DAND1, MGC8972, NB, NO3, differential screening-selected gene aberrant in neuroblastoma, neuroblastoma candidate region, suppression of tumorigenicity 1, neuroblastoma sup

Reactivity

Human

Detection Range

34.4 pg/mL-2200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ITGA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-101
EDTA plasma (n=4)	101	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	100-105
1:2	Average %	94
	Range %	89-98
1:4	Average %	92
	Range %	87-95
1:8	Average %	102
	Range %	94-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.958	1.912	1.935	1.801
10	1.442	1.467	1.455	1.321
5	0.988	0.945	0.967	0.833
2.5	0.562	0.551	0.557	0.423
1.25	0.416	0.423	0.420	0.286
0.625	0.303	0.309	0.306	0.172
0.312	0.236	0.231	0.234	0.100
0	0.133	0.135	0.134	?

Species

Homo sapiens

Sensitivity

8.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	P41271
Antigen	Neuroblastoma, Suppression Of Tumorigenicity 1

Applications

ELISA

Research Area

NBL1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503522	CSB-EL015485HU	96T	$695	Add to Cart
BHE10503522	CSB-EL015485HU	5x96T	$3336	Add to Cart
BHE10503522	CSB-EL015485HU	10x96T	$6602.50	Add to Cart

Human Neuroblastoma suppressor of tumorigenicity 1(NBL1) ELISA kit

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