Human Neural cell adhesion molecule ligand 1,NCAM-L1 ELISA Kit

Human Neural cell adhesion molecule ligand 1,NCAM-L1 ELISA Kit

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Mfr.No.

CSB-E09145h

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

L1 cell adhesion molecule, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, S10, SPG1, OTTHUMP00000025992, OTTHUMP00000063091, antigen identified by monoclonal antibody R1, neural cell adhesion molecule L1

Recovery

The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	104	101-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	93
	Range %	90-96
1:4	Average %	87
	Range %	83-90
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
300	2.278	2.304	2.291	2.111
150	1.422	1.500	1.461	1.281
75	0.932	0.921	0.927	0.747
37.5	0.623	0.624	0.624	0.444
18.75	0.385	0.394	0.390	0.210
9.38	0.287	0.275	0.281	0.101
4.69	0.242	0.231	0.237	0.057
0	0.181	0.179	0.180

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P32004

Applications	ELISA
Research Area	L1CAM

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10503516	CSB-E09145h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.