Human Netrin Receptor Dcc

Mfr.No.

E4180hu

Sensitivity

11.13ng/L

Detection Range

20-6000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Netrin receptor DCC (also known as DCC) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Colorectal cancer suppressor;DCC;Immunoglobulin superfamily DCC subclass member 1;Netrin receptor DCC;Tumor suppressor protein DCC

Molecular Weight

158457

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4180hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1994	77.59	3.9
Intra-Assay	2	18	1354.8	65.97	4.9
Intra-Assay	3	18	690.6	38.43	5.6

Background

Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.

Protocol

These standard curves of E4180hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.112	2.182	2.15
	2.251
1600ng/L	1.428	1.439	1.407
	1.45
800ng/L	0.705	0.672	0.64
	0.639
400ng/L	0.367	0.382	0.35
	0.396
200ng/L	0.185	0.164	0.132
	0.143
0ng/L	0.038	0.032	0
	0.025

Gene ID	1630
UniProt ID	P43146

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105580	E4180hu	48T	$320		Add to Cart
BHE12105580	E4180hu	96T	$458		Add to Cart

Human Netrin Receptor Dcc - DCC ELISA Kit

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