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Home / ELISA Kit

Human Nesprin-2 (SYNE2) ELISA Kit

SKU : BHE13702632

SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. SYNE2 contains multiple spectrin repeats and a 60-amino acid C-terminal region homologous to the Drosophila protein Klarsicht. RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alternative splicing patterns are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif.

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Mfr.No.	KTE60415
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Nesprin-2 (SYNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNE2.
Alternative Names	SYNE2; DKFZp434H2235; DKFZp686E01115; DKFZp686H1931; EDMD5; FLJ11014; FLJ43727; FLJ45710; FLJ46790; KIAA1011; NUA; NUANCE; Nesprin-2; SYNE-2; nesprin 2; nucleus and actin connecting element; synaptic nuclei
Detection Method	Colorimetric
Kit Components	Human Nesprin-2 microplate Human Nesprin-2 standard Human Nesprin-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Nesprin-2 (SYNE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYNE2. No significant cross-reactivity or interference between Human SYNE2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. SYNE2 contains multiple spectrin repeats and a 60-amino acid C-terminal region homologous to the Drosophila protein Klarsicht. RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alteative splicing pattes are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif.
Notes	This Human Nesprin-2 (SYNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNE2 in samples. An antibody specific for SYNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23224

Applications	ELISA
Applications Notes	This Human Nesprin-2 (SYNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNE2 in samples. An antibody specific for SYNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702632	KTE60415	48 T	$339		Add to Cart
BHE13702632	KTE60415	96 T	$589		Add to Cart
BHE13702632	KTE60415	96 Tx5	$2629		Add to Cart
BHE13702632	KTE60415	96 Tx50	$24569		Add to Cart

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