Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Mfr.No.

EK1138

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.032	0.086	0.106	0.196	0.432	0.755	1.370	2.094

Description

Human Nesfatin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NUCB2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Nucleobindin-2;DNA-binding protein NEFA;Gastric cancer antigen Zg4;Prepronesfatin;Nesfatin-1;NUCB2;NEFA;

Molecular Weight

50196 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V25-L106

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CCL8

Subcellular Localization

Golgi apparatus . Membrane ; Peripheral membrane protein . Cytoplasm . Secreted . Endoplasmic reticulum . Nucleus envelope . Golgi retention is mediated by its N-terminal region.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	58	278	993	62	286	1045
Standard deviation	2.55	17.51	57.20	3.41	20.59	74.19
CV (%)	4.4%	7.2%	5.6%	5.5%	7.2%	7.1%

Protein Function

Calcium-binding protein. May have a role in calcium homeostasis.

Background

Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	58	50	49	55	53	3.67	6.9%
Sample 2	278	249	272	250	262	12.93	4.9%
Sample 3	993	1004	914	846	939	64.06	6.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4925
Gene Name	NUCB2
UniProt ID	P80303
Protein Name	Nucleobindin-2
Tissue Specificity	Predominantly expressed in spleen, testis and normal stomach. .
Sequence	Belongs to the nucleobindin family.

Applications	ELISA
Research Area	Calcium Binding Proteins,Calcium Signaling,Chromatin Binding Proteins,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000786	EK1138	96 wells/kit	$499		Add to Cart

Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Rate it now..!!