Human Nerve growth factor,NGF ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04683h

Reactivity

Human

Sample Type

Serum, Tissue Homogenates, Plasma, Cell Culture Supernates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human Rock-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-101
EDTA plasma (n=4)	96	86-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Rock-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-103
1:2	Average %	94
	Range %	86-103
1:4	Average %	95
	Range %	86-102
1:8	Average %	88
	Range %	83-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	P01138
Antigen	nerve growth factor (beta polypeptide)

Applications

ELISA

Research Area

NGF

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503499	CSB-E04683h	10x96T	$5842.50	Add to Cart