Human Nephrin - NPHS1 ELISA Kit

Mfr.No.

E1091Hu

Sensitivity

0.04ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Nephrin (also known as NPHN) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

CNF;nephrin;NPHN;Nphs1;Renal glomerulus-specific cell adhesion receptor

Molecular Weight

134742

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1091Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.64	0.5	3.44
Intra-Assay	2	18	6.05	0.36	6.02
Intra-Assay	3	18	4.15	0.3	7.28

Background

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

Protocol

These standard curves of E1091Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.049	2.116	2.072
	2.183
12ng/ml	1.267	1.243	1.199
	1.218
6ng/ml	0.731	0.759	0.715
	0.787
3ng/ml	0.482	0.488	0.444
	0.493
1.5ng/ml	0.234	0.21	0.167
	0.186
0ng/ml	0.059	0.044	0
	0.028

Gene ID	4868
UniProt ID	O60500

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102743	E1091Hu	48T	$320		Add to Cart
BHE12102743	E1091Hu	96T	$458		Add to Cart

Human Nephrin - NPHS1 ELISA Kit

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