Human Nephrin ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09886h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of rat CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-91
1:2	Average %	90
	Range %	85-95
1:4	Average %	97
	Range %	92-103
1:8	Average %	105
	Range %	100-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

UniProt ID	O60500

Applications	ELISA
Research Area	NPHS1

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10503495	CSB-E09886h	96T	$790	Add to Cart
BHE10503495	CSB-E09886h	5x96T	$3792	Add to Cart
BHE10503495	CSB-E09886h	10x96T	$7505	Add to Cart