Matrices listed below were spiked with certain level of Neopterin and the recovery rates were calculated by comparing the measured value to the expected amount of Neopterin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
94
EDTA plasma(n=5)
88-104
96
heparin plasma(n=5)
90-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neopterin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Neopterin ELISA Kit allows for the in vitro quantitative determination of Neopterin concentrations in serum, plasma, tissue homogenates and other biological fluids.