Human Neogenin(NEO1) ELISA kit

Human Neogenin(NEO1) ELISA kit

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Mfr.No.

CSB-EL015712HU

Alternative Names

neogenin homolog 1 (chicken), DKFZp547A066, DKFZp547B146, HsT17534, IGDCC2, NGN, immunoglobulin superfamily, DCC subclass, member 2, neogenin homolog 1

Reactivity

Human

Detection Range

78 pg/mL-5000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ITGB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	95
	Range %	90-105
1:4	Average %	103
	Range %	97-108
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.433	2.579	2.506	2.362
4000	1.887	1.936	1.912	1.768
2000	1.406	1.488	1.447	1.303
1000	0.949	1.015	0.982	0.838
500	0.635	0.669	0.652	0.508
250	0.371	0.345	0.358	0.214
125	0.262	0.247	0.255	0.111
0	0.140	0.148	0.144

Species

Homo sapiens

Sensitivity

19.5 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q92859
Antigen	neogenin homolog 1 (chicken)

Applications

ELISA

Research Area

NEO1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503494	CSB-EL015712HU	96T	$695	Add to Cart
BHE10503494	CSB-EL015712HU	5x96T	$3336	Add to Cart
BHE10503494	CSB-EL015712HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.