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Home / ELISA Kit

Human Negative elongation factor C/D (TH1L) ELISA Kit

SKU : BHE13702736

The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. Negative elongation factor C/D (TH1L) is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.

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Mfr.No.	KTE60520
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Negative elongation factor C/D (TH1L) ELISA Kit employs a two-site sandwich ELISA to quantitate TH1L.
Alternative Names	TH1L; HSPC130; NELF-C; NELF-D; TH1; TH1 drosophila homolog; TH1-like protein; negative elongation factor proteins C and D; trihydrophobin 1
Detection Method	Colorimetric
Kit Components	Human Negative elongation factor C/D microplate Human Negative elongation factor C/D standard Human Negative elongation factor C/D detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Negative elongation factor C/D (TH1L) ELISA Kit has high sensitivity and excellent specificity for detection of Human TH1L. No significant cross-reactivity or interference between Human TH1L and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. Negative elongation factor C/D (TH1L) is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.
Notes	This Human Negative elongation factor C/D (TH1L) ELISA Kit employs a two-site sandwich ELISA to quantitate TH1L in samples. An antibody specific for TH1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTH1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	51497

Applications	ELISA
Applications Notes	This Human Negative elongation factor C/D (TH1L) ELISA Kit employs a two-site sandwich ELISA to quantitate TH1L in samples. An antibody specific for TH1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTH1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702736	KTE60520	48 T	$339		Add to Cart
BHE13702736	KTE60520	96 T	$589		Add to Cart
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BHE13702736	KTE60520	96 Tx50	$24569		Add to Cart

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