Human NEF3(Neurofilament 3) ELISA Kit

Mfr.No.

EH3412

Alternative Names

NEF3 ELISA Kit, Neurofilament 3 ELISA Kit, NF-M ELISA Kit, NFM ELISA Kit, Neurofilament ELISA Kit, medium polypeptide ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

15.625-1000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of NEF3 and the recovery rates were calculated by comparing the measured value to the expected amount of NEF3 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-105	94
EDTA plasma(n=5)	88-103	97
heparin plasma(n=5)	85-103	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEF3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-102%	89-105%	86-101%
EDTA plasma(n=5)	85-99%	82-100%	88-97%
heparin plasma(n=5)	81-99%	80-100%	82-99%

Sensitivity

< 9.375pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

NEF3/Neurofilament 3/NF-M/NFM/Neurofilament, medium polypeptide

Notes

For Research Use Only

UniProt ID	P07197
Antigen	NEF3

Applications	ELISA
Applications Notes	NEF3 ELISA Kit allows for the in vitro quantitative determination of NEF3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10803063	EH3412	96T	$400		Add to Cart

Human NEF3(Neurofilament 3) ELISA Kit

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