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Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit

SKU : BHE13702236

WWP2 encodes a member of the NEDD4-like protein family. The family of proteins is known to possess ubiquitin-protein ligase activity. The encoded protein contains 4 tandem WW domains. The WW domain is a protein motif consisting of 35 to 40 amino acids and is characterized by 4 conserved aromatic residues. The WW domain may mediate specific protein-protein interactions. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.The deduced 870-amino acid protein has a calculated molecular mass of 98.7 kD and shares 96% identity with human WWP2. Northern blot analysis detected 2 Wwp2 transcripts, with highest expression in testis, followed by spleen, kidney, and liver.

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Mfr.No.	KTE60016
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit employs a two-site sandwich ELISA to quantitate WWP2.
Alternative Names	WWP2; AIP2; WWp2-like; Nedd-4-like ubiquitin-protein ligase; OTTHUMP00000174895; WW domain-containing protein 2; atrophin-1 interacting protein 2
Detection Method	Colorimetric
Kit Components	Human NEDD4-like E3 ubiquitin-protein ligase WWP2 microplate Human NEDD4-like E3 ubiquitin-protein ligase WWP2 standard Human NEDD4-like E3 ubiquitin-protein ligase WWP2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human WWP2. No significant cross-reactivity or interference between Human WWP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	WWP2 encodes a member of the NEDD4-like protein family. The family of proteins is known to possess ubiquitin-protein ligase activity. The encoded protein contains 4 tandem WW domains. The WW domain is a protein motif consisting of 35 to 40 amino acids and is characterized by 4 conserved aromatic residues. The WW domain may mediate specific protein-protein interactions. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.The deduced 870-amino acid protein has a calculated molecular mass of 98.7 kD and shares 96% identity with human WWP2. Northern blot analysis detected 2 Wwp2 transcripts, with highest expression in testis, followed by spleen, kidney, and liver.
Notes	This Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit employs a two-site sandwich ELISA to quantitate WWP2 in samples. An antibody specific for WWP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWWP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WWP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WWP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11060

Applications	ELISA
Applications Notes	This Human NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) ELISA Kit employs a two-site sandwich ELISA to quantitate WWP2 in samples. An antibody specific for WWP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWWP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WWP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WWP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702236	KTE60016	48 T	$339		Add to Cart
BHE13702236	KTE60016	96 T	$589		Add to Cart
BHE13702236	KTE60016	96 Tx5	$2629		Add to Cart
BHE13702236	KTE60016	96 Tx50	$24569		Add to Cart

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