Human Nectin-4/PVRL4 ELISA Kit PicoKine®

Mfr.No.

EK1536

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.026	0.141	0.257	0.469	0.82	1.336	1.832	2.182

Description

Human Nectin-4/PVRL4 ELISA Kit PicoKine® (96 Tests). Quantitate Human NECTIN4 in cell culture supeatants, serum, plasma(heparin, EDTA), cell lysates and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Nectin-4;Ig superfamily receptor LNIR;Nectin cell adhesion molecule 4 ;Poliovirus receptor-related protein 4;Processed poliovirus receptor-related protein 4;NECTIN4 ;LNIR, PRR4, PVRL4;

Molecular Weight

55454 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G32-S349

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Nectin-4

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Cell junction, adherens junction . Colocalizes with MLLT4 at cadherin-based adherens junctions (PubMed:11544254).

Sample Type

Cell culture supeatants, Serum, Plasma, Cell lysates, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	75	209	857	70	228	777
Standard deviation	3.67	13.16	44.56	4.13	16.41	41.18
CV (%)	4.9%	7.2%%	5.2%	5.9%	7.2%	5.3%

Protein Function

Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.

Background

Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alteatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	75	82	86	80	80	3.96	4.9%
Sample 2	209	190	207	207	203	7.69	3.7%
Sample 3	857	1006	836	933	908	67.1	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	81607
Gene Name	NECTIN4
UniProt ID	Q96NY8
Protein Name	Nectin-4
Tissue Specificity	Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. .

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001116	EK1536	96 wells/kit	$499		Add to Cart

Human Nectin-4/PVRL4 ELISA Kit PicoKine®

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