Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of NDUFS1 and the recovery rates were calculated by comparing the measured value to the expected amount of NDUFS1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
95
EDTA plasma(n=5)
87-102
91
heparin plasma(n=5)
88-98
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NDUFS1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
89-104%
90-101%
EDTA plasma(n=5)
85-101%
88-101%
84-99%
heparin plasma(n=5)
83-100%
86-99%
84-94%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P28331
Applications
ELISA
Applications Notes
NDUFS1 ELISA Kit allows for the in vitro quantitative determination of NDUFS1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human NDUFS1 (NADH-ubiquinone oxidoreductase core subunit S1) ELISA Kit