Human Natural Resistance-associated Macrophage Protein 1

Mfr.No.

E4411hu

Sensitivity

7.46ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Natural Resistance-associated Macrophage Protein 1 (also known as SLC11A1/NRAMP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

LSH;Natural resistance-associated macrophage protein 1;NRAMP;NRAMP 1;NRAMP1;SLC11A1;Solute carrier family 11 member 1

Molecular Weight

59872

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4411hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1029.92	32.47	3.15
Intra-Assay	2	18	928.66	34.52	3.72
Intra-Assay	3	18	500.16	24.16	4.83

Background

Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.

Protocol

These standard curves of E4411hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.76	1.756	1.724
	1.753
800ng/L	0.944	0.977	0.945
	1.011
400ng/L	0.446	0.461	0.429
	0.476
200ng/L	0.278	0.27	0.237
	0.261
100ng/L	0.114	0.087	0.055
	0.061
0ng/L	0.028	0.032	0
	0.037

Gene ID	6556
UniProt ID	P49279

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105762	E4411hu	48T	$320		Add to Cart
BHE12105762	E4411hu	96T	$458		Add to Cart

Human Natural Resistance-associated Macrophage Protein 1 - SLC11A1 ELISA Kit

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