Matrices listed below were spiked with certain level of NANOG and the recovery rates were calculated by comparing the measured value to the expected amount of NANOG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
96
EDTA plasma(n=5)
88-104
96
heparin plasma(n=5)
89-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NANOG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NANOG ELISA Kit allows for the in vitro quantitative determination of NANOG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human NANOG(Homeobox protein NANOG) ELISA Kit