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Home / ELISA Kit

Human NADH-ubiquinone oxidoreductase chain 4 (MT-ND4) ELISA Kit

SKU : BHE13703703

MT-ND4 (Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 4) is a Protein Coding gene. Diseases associated with MT-ND4 include Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, And Stroke-Like Episodes and Leber Optic Atrophy. Among its related pathways are Metabolism and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.. GO annotations related to this gene include NADH dehydrogenase (ubiquinone) activity.

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Mfr.No.	KTE61488
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human NADH-ubiquinone oxidoreductase chain 4 (MT-ND4) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND4.
Alternative Names	MT-ND4; MTND4; NADH dehydrogenase subunit 4
Detection Method	Colorimetric
Kit Components	Human NADH-ubiquinone oxidoreductase chain 4 microplate Human NADH-ubiquinone oxidoreductase chain 4 standard Human NADH-ubiquinone oxidoreductase chain 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human NADH-ubiquinone oxidoreductase chain 4 (MT-ND4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT-ND4. No significant cross-reactivity or interference between Human MT-ND4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MT-ND4 (Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 4) is a Protein Coding gene. Diseases associated with MT-ND4 include Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, And Stroke-Like Episodes and Leber Optic Atrophy. Among its related pathways are Metabolism and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.. GO annotations related to this gene include NADH dehydrogenase (ubiquinone) activity.
Notes	This Human NADH-ubiquinone oxidoreductase chain 4 (MT-ND4) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND4 in samples. An antibody specific for MT-ND4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4538

Applications	ELISA
Applications Notes	This Human NADH-ubiquinone oxidoreductase chain 4 (MT-ND4) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND4 in samples. An antibody specific for MT-ND4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703703	KTE61488	48 T	$339		Add to Cart
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