Human Nadh-ubiquinone Oxidoreductase Chain 2

Mfr.No.

E5735Hu

Sensitivity

9.04ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MT ND 2;MT ND2;MT-ND2;NADH dehydrogenase subunit 2;NADH-ubiquinone oxidoreductase chain 2

Molecular Weight

38961

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5735Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1978.7	69.32	3.5
Intra-Assay	2	18	1147.9	51.14	4.5
Intra-Assay	3	18	709	33.71	4.8

Background

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

Protocol

These standard curves of E5735Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.906	1.958	1.918
	2.011
1200ng/L	0.974	1.051	1.011
	1.128
600ng/L	0.39	0.465	0.424
	0.54
300ng/L	0.221	0.251	0.211
	0.281
150ng/L	0.093	0.126	0.086
	0.16
0ng/L	0.038	0.04	0
	0.043

Gene ID	4536
UniProt ID	P03891

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107097	E5735Hu	48T	$320		Add to Cart
BHE12107097	E5735Hu	96T	$458		Add to Cart

Human Nadh-ubiquinone Oxidoreductase Chain 2 - MT-ND2 ELISA Kit

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