Human NADH-cytochrome b5 reductase 3(CYB5R3) ELISA kit

Human NADH-cytochrome b5 reductase 3(CYB5R3) ELISA kit

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Mfr.No.

CSB-EL006320HU

Alternative Names

cytochrome b5 reductase 3, B5R, DIA1, NADH-cytochrome b5 reductase, OTTHUMP00000198574, OTTHUMP00000198665, diaphorase (NADH) (cytochrome b-5 reductase)

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Duration

1-5h

Recovery

The recovery of mouse NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	109
	Range %	104-113
1:2	Average %	93
	Range %	89-99
1:4	Average %	100
	Range %	96-104
1:8	Average %	88
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.779	2.798	2.789	2.587
5	2.201	2.156	2.179	1.977
2.5	1.484	1.451	1.468	1.266
1.25	0.845	0.899	0.872	0.670
0.625	0.555	0.561	0.558	0.356
0.312	0.432	0.445	0.439	0.237
0.156	0.312	0.326	0.319	0.117
0	0.201	0.203	0.202

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P00387
Antigen	cytochrome b5 reductase 3

Applications

ELISA

Research Area

CYB5R3

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503478	CSB-EL006320HU	96T	$695	Add to Cart
BHE10503478	CSB-EL006320HU	5x96T	$3336	Add to Cart
BHE10503478	CSB-EL006320HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.