Human NAD (P)H Dehydrogenase, Quinone 1 (NQO1)ELISA Kit

Human NAD (P)H Dehydrogenase, Quinone 1 (NQO1)ELISA Kit

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Mfr.No.

CSB-E14923h

Sensitivity

9.75 pg/mL

Detection Range

39 pg/mL-2500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

NAD(P)H dehydrogenase, quinone 1, DHQU, DIA4, DTD, NMOR1, NMORI, QR1, DT-diaphorase, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH)

Recovery

The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	101
	Range %	92-110
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.840	2.785	2.813	2.710
500	2.316	2.223	2.270	2.167
250	1.580	1.567	1.574	1.471
125	0.935	0.874	0.905	0.802
62.5	0.479	0.462	0.471	0.368
31.2	0.266	0.254	0.260	0.157
15.6	0.170	0.158	0.164	0.061
0	0.107	0.099	0.103	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P15559

Applications	ELISA
Research Area	NQO1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503482	CSB-E14923h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.