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Home / ELISA Kit

Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit

SKU : BHE13702868

SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. A set of crystal structures of human SIRT3 have been solved, including an apo-structure with no substrate, a structure with a peptide containing acetyl lysine of its natural substrate acetyl-CoA synthetase 2, a reaction intermediate structure trapped by a thioacetyl peptide and a structure with the dethioacetylated peptide bound. These structures show the conformational changes induced by the two substrates required for the reaction, the acetylated substrate peptide and NAD+. In addition, a binding study by isothermal titration calorimetry suggests that the acetylated peptide is the first substrate to bind to SIRT3, prior to NAD+.

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Mfr.No.	KTE60652
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT3.
Alternative Names	SIRT3; SIR2L3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; silent mating type information regulation 2; S.cerevisiae; homolog 3; sir2-like 3; sirtuin 3; sirtuin type 3
Detection Method	Colorimetric
Kit Components	Human NAD-dependent deacetylase sirtuin-3, mitochondrial microplate Human NAD-dependent deacetylase sirtuin-3, mitochondrial standard Human NAD-dependent deacetylase sirtuin-3, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRT3. No significant cross-reactivity or interference between Human SIRT3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. A set of crystal structures of human SIRT3 have been solved, including an apo-structure with no substrate, a structure with a peptide containing acetyl lysine of its natural substrate acetyl-CoA synthetase 2, a reaction intermediate structure trapped by a thioacetyl peptide and a structure with the dethioacetylated peptide bound. These structures show the conformational changes induced by the two substrates required for the reaction, the acetylated substrate peptide and NAD+. In addition, a binding study by isothermal titration calorimetry suggests that the acetylated peptide is the first substrate to bind to SIRT3, prior to NAD+.
Notes	This Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT3 in samples. An antibody specific for SIRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23410

Applications	ELISA
Applications Notes	This Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT3 in samples. An antibody specific for SIRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702868	KTE60652	48 T	$339		Add to Cart
BHE13702868	KTE60652	96 T	$589		Add to Cart
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BHE13702868	KTE60652	96 Tx50	$24569		Add to Cart

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