Human NACHT, LRR and PYD domains-containing protein 1(NLRP1) ELISA kit

Mfr.No.

CSB-EL015864HU

Sensitivity

4.67 pg/mL

Detection Range

18.75 pg/mL-1200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

NLR family, pyrin domain containing 1, CARD7, CLR17.1, DEFCAP, DEFCAP-L/S, DKFZp586O1822, KIAA0926, NAC, NALP1, PP1044, SLEV1, VAMAS1, NACHT, LRR and PYD containing protein 1, NACHT, leucine rich repeat and PYD (pyrin domain) containing 1

Recovery

The recovery of rat IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	98	93-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-93
1:2	Average %	94
	Range %	90-102
1:4	Average %	98
	Range %	94-102
1:8	Average %	95
	Range %	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.201	2.321	2.261	2.068
200	1.678	1.750	1.714	1.521
100	1.143	1.165	1.154	0.961
50	0.746	0.748	0.747	0.554
25	0.439	0.468	0.454	0.261
12.5	0.298	0.302	0.300	0.107
6.25	0.245	0.255	0.250	0.057
0	0.188	0.198	0.193	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9C000

Applications	ELISA
Research Area	NLRP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503469	CSB-EL015864HU	96T	$695	Add to Cart
BHE10503469	CSB-EL015864HU	5x96T	$3336	Add to Cart
BHE10503469	CSB-EL015864HU	10x96T	$6602.50	Add to Cart

Human NACHT, LRR and PYD domains-containing protein 1(NLRP1) ELISA kit

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