Human Nacht - Lrr and Pyd Domains-containing Pprotein 2

Mfr.No.

E4050hu

Description

This sandwich kit is for the accurate quantitative detection of human NACHT,LRR and PYD Domains-containing Protein 2 (also known as NLRP2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CLR19.9;NACHT, LRR and PYD domains-containing protein 2;NALP2;NBS1;NLRP2;Nucleotide-binding site protein 1;PAN1;PYPAF2;PYRIN domain and NACHT domain-containing protein 1;PYRIN-containing APAF1-like protein 2

Reactivity

Human

Detection Range

10-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

120515

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.12ng/L

Background

Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.

Gene ID	55655
UniProt ID	Q9NX02
Antigen	NLRP2

Research Area

Cell Biology

Protocol

These standard curves of E4050hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.358	2.297	2.256
	2.237
800ng/L	1.703	1.718	1.677
	1.733
400ng/L	1.226	1.264	1.223
	1.302
200ng/L	0.696	0.652	0.611
	0.607
100ng/L	0.416	0.423	0.382
	0.43
0ng/L	0.051	0.041	0
	0.031

Precision

We measured random samples of E4050hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	379.96	25.71	6.77
Intra-Assay	2	18	743.26	32.37	4.35
Intra-Assay	3	18	173.91	10.3	5.92

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105469	E4050hu	48T	$320		Add to Cart
BHE12105469	E4050hu	96T	$458		Add to Cart

Human Nacht - Lrr and Pyd Domains-containing Pprotein 2 - NLRP2 ELISA Kit

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