Matrices listed below were spiked with certain level of N-MID-OT and the recovery rates were calculated by comparing the measured value to the expected amount of N-MID-OT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-104
99
EDTA plasma(n=5)
88-99
95
heparin plasma(n=5)
89-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-MID-OT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
N-MID-OT ELISA Kit allows for the in vitro quantitative determination of N-MID-OT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human N-MID-OT(N-MID Osteocalcin) ELISA Kit