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Home / ELISA Kit

Human N-acetyltransferase 14 (NAT14) ELISA Kit

SKU : BHE13703583

NAT14 (N-Acetyltransferase 14 (Putative)) is a Protein Coding gene. Among its related pathways are Cytochrome P450 - arranged by substrate type. GO annotations related to this gene include N-acetyltransferase activity. Probable acetyltransferase that binds the 5-GGACTACAG-3 sequence of coproporphyrinogen oxidase promoter. Able to activate transcription of a reporter construct in vitro.

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Mfr.No.	KTE61368
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human N-acetyltransferase 14 (NAT14) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT14.
Alternative Names	NAT14; KLP1; K562 cell-derived leucine-zipper-like protein 1; K562 cells-derived leucine zipper-like protein 1; K562 cells-derived leucine-zipper-like protein 1; N-acetyltransferase 14
Detection Method	Colorimetric
Kit Components	Human N-acetyltransferase 14 microplate Human N-acetyltransferase 14 standard Human N-acetyltransferase 14 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human N-acetyltransferase 14 (NAT14) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAT14. No significant cross-reactivity or interference between Human NAT14 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	NAT14 (N-Acetyltransferase 14 (Putative)) is a Protein Coding gene. Among its related pathways are Cytochrome P450 - arranged by substrate type. GO annotations related to this gene include N-acetyltransferase activity. Probable acetyltransferase that binds the 5-GGACTACAG-3 sequence of coproporphyrinogen oxidase promoter. Able to activate transcription of a reporter construct in vitro.
Notes	This Human N-acetyltransferase 14 (NAT14) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT14 in samples. An antibody specific for NAT14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAT14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57106

Applications	ELISA
Applications Notes	This Human N-acetyltransferase 14 (NAT14) ELISA Kit employs a two-site sandwich ELISA to quantitate NAT14 in samples. An antibody specific for NAT14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAT14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703583	KTE61368	48 T	$339		Add to Cart
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BHE13703583	KTE61368	96 Tx50	$24569		Add to Cart

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