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Home / ELISA Kit

Human Myotrophin (MTPN) ELISA Kit

SKU : BHE13703694

The transcript produced from MTPN is bi-cistronic and can encode both myotrophin and leucine zipper protein 6. The myotrophin protein is associated with cardiac hypertrophy, where it is involved in the conversion of NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers. Myotrophin also has a potential function in cerebellar morphogenesis, and it may be involved in the differentiation of cerebellar neurons, particularly of granule cells. A cryptic ORF at the 3' end of this transcript uses a novel internal ribosome entry site and a non-AUG translation initiation codon to produce leucine zipper protein 6, a 6.4 kDa tumor antigen that is associated with myeloproliferative disease.

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Mfr.No.	KTE61479
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myotrophin (MTPN) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPN.
Alternative Names	MTPN; FLJ31098; FLJ99857; GCDP; V-1; granule cell differentiation protein
Detection Method	Colorimetric
Kit Components	Human Myotrophin microplate Human Myotrophin standard Human Myotrophin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myotrophin (MTPN) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTPN. No significant cross-reactivity or interference between Human MTPN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The transcript produced from MTPN is bi-cistronic and can encode both myotrophin and leucine zipper protein 6. The myotrophin protein is associated with cardiac hypertrophy, where it is involved in the conversion of NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers. Myotrophin also has a potential function in cerebellar morphogenesis, and it may be involved in the differentiation of cerebellar neurons, particularly of granule cells. A cryptic ORF at the 3' end of this transcript uses a novel internal ribosome entry site and a non-AUG translation initiation codon to produce leucine zipper protein 6, a 6.4 kDa tumor antigen that is associated with myeloproliferative disease.
Notes	This Human Myotrophin (MTPN) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPN in samples. An antibody specific for MTPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTPN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	136319

Applications	ELISA
Applications Notes	This Human Myotrophin (MTPN) ELISA Kit employs a two-site sandwich ELISA to quantitate MTPN in samples. An antibody specific for MTPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTPN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703694	KTE61479	48 T	$339		Add to Cart
BHE13703694	KTE61479	96 T	$589		Add to Cart
BHE13703694	KTE61479	96 Tx5	$2629		Add to Cart
BHE13703694	KTE61479	96 Tx50	$24569		Add to Cart

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