Human Myostatin,MSTN ELISA Kit

Mfr.No.

CSB-E11300h

Sensitivity

0.312 ng/mL

Detection Range

0.625 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

myostatin, GDF8, growth differentiation factor 8

Recovery

The recovery of human CSTB/CST6/STFB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99-108
EDTA plasma (n=4)	98	93-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CSTB/CST6/STFB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-106
1:2	Average %	91
	Range %	88-97
1:4	Average %	96
	Range %	91-104
1:8	Average %	89
	Range %	84-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O14793

Applications	ELISA
Research Area	MSTN

Storage	Store at 2-8°C. Please refer to protocol.

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