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Home / ELISA Kit

Human Myosin-XVI (MYO16) ELISA Kit

SKU : BHE13703645

MYO16 encodes an unconventional myosin protein. Myosin-XVI has been proposed to act as a serine/threonine phosphatase-1 targeting or regulatory subunit. Studies in a rat cell line suggest that this protein may regulate cell cycle progression. A variant within MYO16 may be associated with susceptibility to schizophrenia and elevated expression of MYO16 has been observed in the frontal cortex of human schizophrenia patients.

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Mfr.No.	KTE61430
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myosin-XVI (MYO16) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO16.
Alternative Names	MYO16; RP11-54H7.1; KIAA0865; MYR8; Myo16b; MYO16 variant protein; OTTHUMP00000018693; myosin heavy chain Myr 8
Detection Method	Colorimetric
Kit Components	Human Myosin-XVI microplate Human Myosin-XVI standard Human Myosin-XVI detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myosin-XVI (MYO16) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO16. No significant cross-reactivity or interference between Human MYO16 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYO16 encodes an unconventional myosin protein. Myosin-XVI has been proposed to act as a serine/threonine phosphatase-1 targeting or regulatory subunit. Studies in a rat cell line suggest that this protein may regulate cell cycle progression. A variant within MYO16 may be associated with susceptibility to schizophrenia and elevated expression of MYO16 has been observed in the frontal cortex of human schizophrenia patients.
Notes	This Human Myosin-XVI (MYO16) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO16 in samples. An antibody specific for MYO16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23026

Applications	ELISA
Applications Notes	This Human Myosin-XVI (MYO16) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO16 in samples. An antibody specific for MYO16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703645	KTE61430	48 T	$339		Add to Cart
BHE13703645	KTE61430	96 T	$589		Add to Cart
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