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Home / ELISA Kit

Human Myosin-Va (MYO5A) ELISA Kit

SKU : BHE13703631

MYO5A is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. Myosin-Va encoded by MYO5A is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.

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Mfr.No.	KTE61416
Description	This Human Myosin-Va (MYO5A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO5A.
Alternative Names	MYO5A; GS1; MYH12; MYO5; MYR12; dilute; myosin V; myosin VA; myosin; heavy polypeptide kinase; myoxin
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Myosin-Va microplate Human Myosin-Va standard Human Myosin-Va detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myosin-Va (MYO5A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO5A. No significant cross-reactivity or interference between Human MYO5A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYO5A is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. Myosin-Va encoded by MYO5A is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.
Notes	This Human Myosin-Va (MYO5A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO5A in samples. An antibody specific for MYO5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4644
Antigen	MYO5A
Tag	MYO5A
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Myosin-Va (MYO5A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO5A in samples. An antibody specific for MYO5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703631	KTE61416	48 T	$339		Add to Cart
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