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Home / ELISA Kit

Human Myosin regulatory light chain 12A (MYL12A) ELISA Kit

SKU : BHE13703660

MYL12A encodes a nonsarcomeric myosin regulatory light chain. Myosin regulatory light chain 12A is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. Myosin regulatory light chain 12A may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

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Mfr.No.	KTE61445
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myosin regulatory light chain 12A (MYL12A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12A.
Alternative Names	MYL12A; MLCB; MRCL3; MRLC3; MYL2B; myosin regulatory light chain 3; myosin regulatory light chain MRCL3; myosin regulatory light chain MRLC3; myosin; light polypeptide; regulatory; non-sarcomeric (20kD)
Detection Method	Colorimetric
Kit Components	Human Myosin regulatory light chain 12A microplate Human Myosin regulatory light chain 12A standard Human Myosin regulatory light chain 12A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myosin regulatory light chain 12A (MYL12A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL12A. No significant cross-reactivity or interference between Human MYL12A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYL12A encodes a nonsarcomeric myosin regulatory light chain. Myosin regulatory light chain 12A is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. Myosin regulatory light chain 12A may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.
Notes	This Human Myosin regulatory light chain 12A (MYL12A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12A in samples. An antibody specific for MYL12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10627

Applications	ELISA
Applications Notes	This Human Myosin regulatory light chain 12A (MYL12A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12A in samples. An antibody specific for MYL12A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703660	KTE61445	48 T	$339		Add to Cart
BHE13703660	KTE61445	96 T	$589		Add to Cart
BHE13703660	KTE61445	96 Tx5	$2629		Add to Cart
BHE13703660	KTE61445	96 Tx50	$24569		Add to Cart

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