Human myosin light chain kinase,MLCK ELISA Kit

Human myosin light chain kinase,MLCK ELISA Kit

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Mfr.No.

CSB-E09048h

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

myosin light chain kinase, DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK, OTTHUMP00000180642, myosin, light polypeptide kinase, smooth muscle myosin light chain kinase

Recovery

The recovery of human CTSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	96	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CTSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	89
	Range %	85-93
1:4	Average %	106
	Range %	102-109
1:8	Average %	94
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.745	2.849	2.797	2.693
500	2.257	2.412	2.335	2.231
250	1.616	1.717	1.667	1.563
125	0.943	0.965	0.954	0.850
62.5	0.547	0.524	0.536	0.432
31.2	0.354	0.363	0.359	0.255
15.6	0.245	0.254	0.250	0.146
0	0.103	0.105	0.104

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q15746

Applications	ELISA
Research Area	MYLK

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Yersinia pseudotuberculosis disrupts intestinal barrier integrity through hematopoietic TLR-2 signaling /,J Clin Invest,Jun 122(6):2239-51.

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BHE10503459	CSB-E09048h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.