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Home / ELISA Kit

Human Myosin light chain 5 (MYL5) ELISA Kit

SKU : BHE13703655

MYL5 encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. Myosin light chain 5, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.

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Mfr.No.	KTE61440
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myosin light chain 5 (MYL5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL5.
Alternative Names	MYL5; myosin regulatory light chain 5; myosin; light polypeptide 5; regulatory; superfast myosin regulatory light chain 2
Detection Method	Colorimetric
Kit Components	Human Myosin light chain 5 microplate Human Myosin light chain 5 standard Human Myosin light chain 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myosin light chain 5 (MYL5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL5. No significant cross-reactivity or interference between Human MYL5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYL5 encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. Myosin light chain 5, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.
Notes	This Human Myosin light chain 5 (MYL5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL5 in samples. An antibody specific for MYL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4636

Applications	ELISA
Applications Notes	This Human Myosin light chain 5 (MYL5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL5 in samples. An antibody specific for MYL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703655	KTE61440	48 T	$339		Add to Cart
BHE13703655	KTE61440	96 T	$589		Add to Cart
BHE13703655	KTE61440	96 Tx5	$2629		Add to Cart
BHE13703655	KTE61440	96 Tx50	$24569		Add to Cart

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