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Home / ELISA Kit

Human Myosin-Ia (MYO1A) ELISA Kit

SKU : BHE13703641

MYO1A encodes a member of the myosin superfamily. Myosin-Ia represents an unconventional myosin; it should not be confused with the conventional skeletal muscle myosin-1 (MYH1). Unconventional myosins contain the basic domains characteristic of conventional myosins and are further distinguished from class members by their tail domains. They function as actin-based molecular motors. Mutations in this gene have been associated with autosomal dominant deafness. Alternatively spliced variants have been found for MYO1A.

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Mfr.No.	KTE61426
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myosin-Ia (MYO1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1A.
Alternative Names	MYO1A; BBMI; DFNA48; MIHC; MYHL; brush border myosin-I; myosin I heavy chain; myosin; heavy polypeptide-like (100kD)
Detection Method	Colorimetric
Kit Components	Human Myosin-Ia microplate Human Myosin-Ia standard Human Myosin-Ia detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myosin-Ia (MYO1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO1A. No significant cross-reactivity or interference between Human MYO1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYO1A encodes a member of the myosin superfamily. Myosin-Ia represents an unconventional myosin; it should not be confused with the conventional skeletal muscle myosin-1 (MYH1). Unconventional myosins contain the basic domains characteristic of conventional myosins and are further distinguished from class members by their tail domains. They function as actin-based molecular motors. Mutations in this gene have been associated with autosomal dominant deafness. Alternatively spliced variants have been found for MYO1A.
Notes	This Human Myosin-Ia (MYO1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1A in samples. An antibody specific for MYO1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4640

Applications	ELISA
Applications Notes	This Human Myosin-Ia (MYO1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1A in samples. An antibody specific for MYO1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703641	KTE61426	48 T	$339		Add to Cart
BHE13703641	KTE61426	96 T	$589		Add to Cart
BHE13703641	KTE61426	96 Tx5	$2629		Add to Cart
BHE13703641	KTE61426	96 Tx50	$24569		Add to Cart

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