Human Myosin-11(MYH11) ELISA kit

Human Myosin-11(MYH11) ELISA kit

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Mfr.No.

CSB-EL015291HU

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

myosin, heavy chain 11, smooth muscle, AAT4, DKFZp686D10126, DKFZp686D19237, FAA4, FLJ35232, MGC126726, MGC32963, SMHC, SMMHC, myosin, heavy polypeptide 11, smooth muscle, smooth muscle myosin heavy chain 11

Recovery

The recovery of mouse IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-107
EDTA plasma (n=4)	98	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-95
1:200	Average %	95
	Range %	91-99
1:400	Average %	97
	Range %	96-105
1:800	Average %	98
	Range %	89-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50	2.695	2.594	2.645	2.454
25	2.004	1.903	1.954	1.763
12.5	1.111	1.204	1.158	0.967
6.25	0.681	0.670	0.676	0.485
3.12	0.441	0.452	0.447	0.256
1.56	0.325	0.315	0.320	0.129
0.78	0.260	0.249	0.255	0.064
0	0.191	0.190	0.191	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35749

Applications	ELISA
Research Area	MYH11

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503454	CSB-EL015291HU	96T	$695	Add to Cart
BHE10503454	CSB-EL015291HU	5x96T	$3336	Add to Cart
BHE10503454	CSB-EL015291HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.